Publications Récentes
2012
A. Abou Hamdan, S. Dementin, P.-P. Liebgott, O. Gutierrez-Sanz, P. Richaud, A. L. De Lacey, M. Rousset, P. Bertrand, L. Cournac and C. Leger, “Understanding and tuning the catalytic bias of hydrogenase” J. Am. Chem. Soc. (2012) doi:10.1021/ja301802r
2011
(document pédagogique) Dispositives d’introduction à la RPE, par Patrick Bertrand. Qu’est ce qu’un spectre RPE? [ppt] [pdf] A quoi sert la RPE? [ppt] [pdf]
S. Dementin, C. Burlat, V. Fourmond, F. Leroux, P.P. Liebgott, A. Abou Hamdan, C. Leger, M. Rousset, B. Guigliarelli, P. Bertrand, “Rates of intra and intermolecular electron transfers in hydrogenase deduced from steady-state activity measurements” J. Am. Chem. Soc. 133 10211 (2011) doi: 10.1021/ja102615a
(document pédagogique) Une introduction (en anglais) à l’électrochimie, de l’équation de Nernst à l’électrochimie directe des protéines, écrite à l’occasion du workshop Chemistry of Metals in Biological Systems, organisé par Robert R. Chrichton et R. Louro à Louvain-la-neuve, Belgique. [pdf]
C Baffert, L Bertini, T Lautier, C Greco, K Sybirna, P Ezanno, E Etienne, P Soucaille, P Bertrand, H Bottin, I Meynial-Salles, L De Gioia, C Leger “CO disrupts the reduced H-cluster of FeFe hydrogenase. A combined DFT and PFV study” J. Am. Chem. Soc. 133 2096-2099 (2011) doi: 10.1021/ja110627b
P-P Liebgott, A L. de Lacey, B Burlat, L Cournac, P Richaud, M Brugna, V M. Fernandez, B Guigliarellia, M Rousset, C Leger, S Dementin “Original design of an oxygen-tolerant [NiFe] hydrogenase : Major effect of a valine-to-cysteine mutation near the active site” J. Am. Chem. Soc. 133 986-997 (2011) doi:10.1021/ja108787s
O. Kolaj-Robin, S. R O’Kane, W. Nitschke, C. Leger, F. Baymann, T. Soulimane. “Biochemical and biophysical characterization of succinate:quinone reductase from Thermus thermophilus” BBA – Bioenergetics, 1807 68-79 (2011) doi:10.1016/j.bbabio.2010.10.009
T. Lautier, P. Ezanno, C. Baffert, V. Fourmond, L. Cournac, Juan C. Fontecilla-Camps, P. Soucaille, P. Bertrand, I. Meynial-Salles, Christophe Leger. “The quest for a functional substrate access tunnel in FeFe hydrogenase” Faraday Discussions, 148, 385-407 (2011). doi:10.1039/c004099c
P.P. liebgott, S. Dementin, C. leger and M. Rousset “Towards engineering O2-tolerance in NiFe hydrogenases” Energy and Environmental Science 4, 33-41 (2011) doi:10.1039/c0ee00093k
2010
(document pédagogique) Patrick Bertrand La spectroscopie de Résonance Paramagnétique Electronique, volume 1 EDP Sciences, collection Grenoble Sciences, (2010).
(document pédagogique) Slides of the September 25th 2010 ISE tutorial on Redox protein and redox enzyme electrochemistry: fundamental studies and applications. Part I. C. Leger on direct electron transfer. [pdf] [ppt] Part II. Phil Bartlett on mediated electron transfer.
M.E. Pandelia, V. Fourmond, P. Tron, E. Lojou, P. Bertrand, C. Leger, M.T. Giudici-Orticoni, W. Lubitz “The Membrane-Bound Hydrogenase I from the Hyperthermophilic Bacterium Aquifex aeolicus: Enzyme Activation, Redox Intermediates and Oxygen Tolerance” J. Am. Chem. Soc. 132(20) 6991-7004 (2010) doi:10.1021/ja910838d
(document pédagogique) C. Leger. Séminaire au Collège de France le 14/04/10, “Des électrodes pour étudier et utiliser les hydrogénases: du mécanisme catalytique à la biopile” dans le cadre des Cours de Marc Fontecave sur La chimie du vivant : enzymes et métalloenzymes, des bio-catalyseurs fascinants [Résumé pdf] [pdf] More teaching material on the english version of this page.
V. Fourmond, P. Infossi, MT Giudici-Orticoni, P. Bertrand, C. Leger “Two-step chronoamperometric method for studying the anaerobic inactivation of an oxygen tolerant NiFe hydrogenase” J. Am. Chem. Soc. 132(13), 4848-4857 (2010) doi:10.1021/ja910685j [pdf]
F. Leroux, PP Liebgott, L. Cournac, P. Richaud, A. Kpebe, B. Burlat, B. Guigliarelli, P. Bertrand, C. Leger, M. Rousset, S. Dementin “Is engineering O2-tolerant hydrogenases just a matter of reproducing the active sites of the naturally occurring O2-resistant enzymes?” Int. J. H2 Energ. 35, 10770-10777 (2010) doi:10.1016/j.ijhydene.2010.02.071
C. Valls, J.F. Colom, C. Baffert, I. Gimbert, M. B. Roncero, J.-C. Sigoillot “Comparing the efficiency of the laccaseNHA and laccaseHBT systems in eucalyptus pulp bleaching” Biochem. Engin. J. 49(3) 401-407 (2010) doi:10.1016/j.bej.2010.02.002
V. Fourmond, B. Burlat, S. Dementin, M. Sabaty, P. Arnoux, E. Etienne, B. Guigliarelli, P. Bertrand, D. Pignol and C. Leger. “Dependence of catalytic activity on driving force in solution assays and protein film voltammetry: insights from the comparison of nitrate reductase mutants” Biochemistry 49(11) 2424-2432 (2010) doi:10.1021/bi902140e [pdf]
V. Fourmond, M. Sabaty, P. Arnoux, P. Bertrand, D. Pignol and C. Leger. “Reassessing the Strategies for Trapping Catalytic Intermediates during Nitrate Reductase Turnover” J. Phys. Chem. B 114(9) 3341-3347 (2010) doi:10.1021/jp911443y [pdf]
P.-P. Liebgott, F. Leroux, B. Burlat, S. Dementin, C. Baffert, Th. Lautier, V. Fourmond, P. Ceccaldi, Ch. Cavazza, I. Meynial-Salles, Ph. Soucaille, J. Fontecilla-Camps, B. Guigliarelli, P. Bertrand, M. Rousset, Ch. Leger. “Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase” Nat. Chem. Biol. 6(1) 63-70 (2010) doi:10.1038/nchembio.276
2009
K. H. Diêp Lê, A. Boussac, B. Frangioni, Ch. Leger, F. Lederer “Interdomain Contacts in Flavocytochrome b2, a Mutational Analysis” Biochemistry, 48, 10803-10809 (2009) doi:10.1021/bi901301r [pdf]
S. Dementin, F. Leroux, L. Cournac, A. De Lacey, A. Volbeda, C. Leger, B. Burlat, N. Martinez, S. Champ, L. Martin, O. Sanganas, M. Haumann, V. Fernandez, B. Guigliarelli, J. Fontecilla-Camps, M. Rousset. “Introduction of Methionines in the Gas Channel Makes [NiFe] Hydrogenase Aero-Tolerant” J. Am. Chem. Soc., 131, 10156-10164 (2009) doi:10.1021/ja9018258 [pdf]
(document pédagogique) P. Bertrand. Séminaire au Collège de France le 8/04/09, “La vie trépidante des électrons dans les enzymes d’oxydoréduction” dans le cadre des Cours de Marc Fontecave sur La chimie du vivant : enzymes et métalloenzymes, des bio-catalyseurs fascinants [Résumé pdf] [ppt] [pdf]
V. Fourmond, T. Lautier, C. Baffert, F. Leroux, P.-P. Liebgott, S. Dementin, M. Rousset, P. Arnoux, D. Pignol, I. Meynial, P. Soucaille, P. Bertrand, C. Leger. “Correcting for electrocatalyst desorption and inactivation in chronoamperometry experiments.” Analytical Chemistry, 81, 1962-2968 (2009) doi:10.1021/ac8025702 [pdf]
V. Fourmond, K. Hoke, H. A. Heering, C. Baffert, F. Leroux, P. Bertrand, C. Leger “SOAS: a free software to analyse electrochemical data and other one-dimensional signals” Bioelectrochemistry, 76, 141-147 (2009) doi:10.1016/j.bioelechem.2009.02.010 [pdf]
2008
V. Fourmond, B. Burlat, S. Dementin, P. Arnoux, M. Sabaty, S. Boiry, B. Guigliarelli, P. Bertrand, D. Pignol and C. Leger “Major Mo(V) EPR signature of Rhodobacter sphaeroides periplasmic nitrate reductase arising from a dead-end species that activates upon reduction. Relation to other molybdoenzymes from the DMSO reductase family.” J. Phys. Chem. B 112, 15478-15486 (2008) doi:10.1021/jp807092y [pdf] [Supplement]
F. Leroux, S. Dementin, B. Burlat, L. Cournac, A. Volbeda, S. Champ, L. Martin, B. Guigliarelli, P. Bertrand, J. Fontecilla-Camps, M. Rousset and C. Leger “Experimental Approaches to Kinetics of Gas Diffusion in Hydrogenase” Proc. Natl. Acad. Sci. USA 105, 11188 (2008) doi:10.1073/pnas.0803689105 [pdf] [Supplement]
C. Leger and P. Bertrand. “Direct electrochemistry of redox enzymes as a tool for mechanistic studies” Chem. Rev. 108, 2379 (2008) doi:10.1021/cr0680742 [html] [pdf] [Supplement] This is part of the July, special issue on Molecular and biomolecular electrochemistry
C. Baffert, M. Demuez, L. Cournac, B. Burlat, B. Guigliarelli, P. Bertrand, L. Girbal and C. Leger. “Hydrogen-activating enzymes: activity does not correlate with oxygen sensitivity” Angewandte Chemie Int. Ed. 47, 2052 (2008) doi:10.1002/anie.200704313 [Supplement]
2007
P. Lanciano, A. Savoyant, S. Grimaldi, A. Magalon, B. Guigliarelli and P. Bertrand. “New Method for the Spin Quantitation of [4Fe-4S]+ Clusters with S=3/2. Application to the FS0 Center of the NarGHI Nitrate Reductase from Escherichia coli” J. Phys. Chem. B 111, 13632 (2007). doi:10.1021/jp075243t [pdf]
P. Bertrand, B. Frangioni, S. Dementin, M. Sabaty, P. Arnoux, B. Guigliarelli, D. Pignol and C. Leger. "Effects of slow substrate binding and release in redox enzymes: theory and application to periplasmic nitrate reductase.’’ J. Phys. Chem. B 111, 10300 (2007). doi:10.1021/jp074340j [pdf]
P. Lanciano, A. Magalon, P. Bertrand, B. Guigliarelli, S. Grimaldi “High-stability semiquinone intermediate in nitrate reductase A (NarGHI) from Escherichia coli is located in a quinol oxidation site close to heme bD” Biochemistry 17, 869 (2007). doi:10.1021/bi700074y
S. Dementin, P. Arnoux, B. Frangioni, S. Grosse, C. Leger, B. Burlat, B. Guigliarelli, M. Sabaty, D. Pignol. “Access to the Active Site of Periplasmic Nitrate Reductase: Insights from Site-Directed Mutagenesis and Zinc Inhibition Studies” Biochemistry 38, 12240 (2007) doi:10.1021/bi700928m [pdf]
M. G. Almeida, Célia M. Silveira, B. Guigliarelli, P. Bertrand, J. J. G. Moura, I. Moura and C. Leger. “A needle in a haystack: the active site of the membrane-bound complex cytochrome c nitrite reductase.” FEBS Letters 581, 284 (2007). doi:10.1016/j.febslet.2006.12.023
2006
S. Dementin, V. Belle, P. Bertrand, B. Guigliarelli, G. Adryanczyk-Perrier, A. Delacey, V. M. Fernandez, M. Rousset and C. Leger. “Changing the ligation of the distal [4Fe4S] cluster in NiFe hydrogenase impairs inter- and intramolecular electron transfers” J. Am. Chem. Soc. 128, 5209 (2006) doi:10.1021/ja060233b [pdf]
C. Leger, F. Lederer, B. Guigliarelli and P. Pertrand, “Electron flow in multicenter enzymes: theory, applications and consequences on the natural design of redox chains” J. Am. Chem. Soc. 128, 180 (2006) doi:10.1021/ja055275z [pdf]
M. Guiral, P. Tron, V. Belle, C. Aubert, C. Leger, B. Guigliarelli and M.-T. Giudici-Orticoni. “Hyperthermostable and oxygen resistant hydrogenases from a hyperthermophilic bacterium Aquifex aeolicus: Physicochemical properties” Int. J. Hydrogen. Energ. 31, 1414 (2006) doi:10.1016/j.ijhydene.2006.06.007
2005
C. More, M. Asso, G. Roger, B. Guigliarelli, J. Caldeira, J. Moura and P. Bertrand, “Study of the Spin-Spin Interactions between the Metal Centers of Desulfovibrio gigas Aldehyde Oxidoreductase: Identification of the Reducible Sites of the [2Fe-2S]1+,2+ Clusters” Biochemistry 44, 11628 (2005) doi:10.1021/bi0510025
2004
C. Leger, S. Dementin, P. Bertrand, M. Rousset and B. Guigliarelli, “Inhibition and aerobic inactivation kinetics of Desulfovibrio fructosovorans NiFe hydrogenase studied by Protein Film voltammetry” J. Am. Chem. Soc. 126, 12162 (2004) doi:10.1021/ja046548d [pdf]
B. Frangioni, P. Arnoux, M. Sabaty, D. Pignol, P. Bertrand, B. Guigliarelli and C. Leger “In Rhodobacter sphaeroides respiratory nitrate reductase, the kinetics of substrate binding favors intramolecular electron transfer” J. Am. Chem. Soc., 126, 1328 (2004) doi:10.1021/ja0384072 [pdf]
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