Site map > InGEcoH Project FR
InGEcoH logo C. acetobutylicum D. vulgaris

Ecological engineering of microbial ecosystems
producing biohydrogen by dark fementation

This is the home page of the project InGEcoH financed by the ANR (Agence National de Recherche, the French National Research Agency), a collaboration between the Laboratoire de Biotechnologie de l’Environnement (LBE, Laboratory of Biotechnology of the Environment), Narbonne, the Laboratoire de Bioénérgétique et Ingénierie des Protéines (BIP, Laboratory of Bioenergetics and Protein Engineering); Marseilles, and the Laboratoire d’Ingénierie des Systèmes Biologiques et des Procédés (LISBP, Laboratory for Engineering of Systems Biology and Procedures), Toulouse.

List of Contents

Coordinator of the project

Dr Jean-Phillippe Steyer, Laboratoire de Biotechnologie de l’Environnement, Avenue des Étangs, 11100 Narbonne, France

Tel. + 33 468 425 178 (steyer@supagro.inra.fr)

Partenaires

Laboratoire de Biotechnologie de l’Environnement
Laboratory of Biotechnology of the Environment
(INRA - UR050)

logo
 [INRA logo]

Address: Avenue des Étangs, 11100 Narbonne, France

Contact: Dr Jean-Phillippe Steyer; tel. + 33 468 425 178 (steyer@supagro.inra.fr)

Group: Jérôme Hamelin, Eric Latrille, Marianne Quémeneur, Yan Rafrafi, Eric Trably

Laboratoire de Bioénergétique et Ingénierie des Protéines
Laboratory of Bioenergetics and Protein Engineering
(CNRS – UPR9036)

logo
 [CNRS logo]

Address: 31 chemin Joseph-Aiguier, B.P. 71, 13402 Marseille Cedex 20 France

Contact: Dr Marie-Thérèse Giudici-Orticoni; tel.+ 33 491 16 45 50 (giudici@ifr88.cnrs-mrs.fr)

Group: Saida Benomar, María Luz Cárdenas, Athel Cornish-Bowden, Marianne Guiral, Marianne Ilbert, Gisèle Leroy

Laboratoire d’Ingénierie des Systèmes Biologiques et des Procédés
Laboratory for Engineering of Systems Biology and Procedures
(UMR INSA/CNRS 5504, UMR INSA/INRA 792)

logo
 [CNRS logo]
 [INRA logo]

Address: 135 avenue de Rangueil, 31077 Toulouse France

Contact: Dr Isabelle Meynial-Salles; tel.+ 33 561 55 94 19 (meynial@insa-toulouse.fr)

Group: Christian Croux, Philippe Soucaille

People

Photographie du personnel Jérôme Jean-Phi Eric L. Athel Yan Eric T. Marianne I. Mariam Marilú Gisèle Marianne G.; Mariianne Q. Saida Isabelle Marie-Thé

The photograph was taken in Narbonne in January 2009. Jérôme Hamelin is shown as an inset. Christian Croix was not present when the photograph was taken. Jérôme Hamelin is shown as an inset.


Summary

Numerous microorganisms can produce hydrogen by dark fermentation. Even though biohydrogen productivities and conversion yields can be useful for industrial purposes, there remain several scientific and technical constraints that need to be addressed. In particular, the presence of alternative metabolic pathways for hydrogen consumption generally results in chronic instability of the biological processes in mixed cultures. To increase the stability and the efficiency of dark fermentative processes, it is now necessary to get a better understanding of the metabolic interaction networks between producing and consuming microorganisms. The InGEcoH project plans to develop an innovative and multidisciplinary approach to ecological engineering, consisting of the design, building and study of synthetic microbial consortia to establish the metabolic networks that exist between microorganisms for further optimization of biohydrogen production. More specifically, this implies

  1. selection and characterization of bacteria with a high potential for biohydrogen production,
  2. building of a synthetic microbial consortium to develop mechanistic models of metabolic interaction networks, and
  3. study and optimization of selected consortia for producing biohydrogen efficiently under real conditions and with high stability.

Finance

logo

Project ANR-08-BIOE-005 started in December 2008 for a period of 36 months; support from the ANR (Agence National de Recherche, the French National Research Agency) amounting to €706380.

Contact information

This is a list of email addresses of the participants:

Mariam Benjdiamariam.benjdia@agencerecherche.fr
Saida Benomarsbenomar@ifr88.cnrs-mrs.fr
María Luz Cárdenascardenas@ifr88.cnrs-mrs.fr
Athel Cornish-Bowdenacornish@ifr88.cnrs-mrs.fr
Christian Crouxchristian.croux@insa-toulouse.fr
Marie-Thérèse Giudici-Orticonigiudici@ifr88.cnrs-mrs.fr
Marianne Guiralguiral@ifr88.cnrs-mrs.fr
Jérôme Hamelinhamelin@supagro.inra.fr
Marianne Ilbertmilbert@ifr88.cnrs-mrs.fr
Eric Latrillelatrille@supagro.inra.fr
Gisèle Leroyleroy@ifr88.cnrs-mrs.fr
Isabelle Meynial-Sallesisabelle.meynial-salles@insa-toulouse.fr
Marianne Quémeneurmarianne.quemeneur@supagro.inra.fr
Yan Rafrafirafrafi@supagro.inra.fr
Jean-Philippe Steyersteyer@supagro.inra.fr
Eric Trablytrably@supagro.inra.fr
Senthil Kumar Velusamysenthilk@insa-toulouse.fr

Events

1 December 2008. Official start to the project

8 January 2009. Planned date for the first meeting in Narbonne: cancelled on account of the heavy snowfall in Marseilles!

21 January 2009. First meeting in Narbonne. The following people were present: Mariam Benjdia, Saida Benomar, María Luz Cárdenas, Athel Cornish-Bowden, Marie-Thérèse Giudici-Orticoni, Marianne Guiral, Jérôme Hamelin, Marianne Ilbert, Eric Latrille, Gisèle Leroy, Isabelle Meynial-Salles, Marianne Quémeneur, Yan Rafrafi, Jean-Phillippe Steyer, Eric Trably.

22 April 2009. Technical meeting (BIP, LBE) in Marseilles on the molecular analysis for following the consortia.

1 July 2009. Meeting in Marseilles for presentation to the partners in the project of the results from the first semester of the project and of the progress in the various tasks. The following people were present (shown in the order they appear in the photograph below, from left to right), together with Gisèle Leroy, who is not in the photograph: Marie-Thérèse Giudici-Orticoni, Eric Latrille, Marianne Quémeneur, Jean-Phillippe Steyer, Eric Trably, Marianne Guiral, Isabelle Meynial-Salles, María Luz Cárdenas, Saida Benomar, Jérôme Hamelin, Yan Rafrafi, Marianne Ilbert, Athel Cornish-Bowden.

After presentation of administrative matters by Eric Trably, there was detailed discussion of the following reports:

Yan Marianne Q.
Saida Athel
  1. Presentation of first results of Yan Rafrafi for his doctoral thesis;
  2. Presentation of preliminary studies by Marianne Quémeneur;
  3. Presentation of first results of Saida Benomar for her doctoral thesis;
  4. Presentation of an example of kinetic modelling by Athel Cornish-Bowden.

Photographie du personnel à Marseille

26 January 2010. Meeting in Toulouse for presentation to the partners in the project of the results from the second semester of the project and of the progress in the various tasks. The following people were present (shown in the order they appear in the photograph below, from left to right): Jean-Phillippe Steyer, Senthil Kumar Velusamy, Isabelle Meynial-Salles, Marianne Quémeneur, Eric Latrille, Yan Rafrafi, María Luz Cárdenas, Athel Cornish-Bowden, Jérôme Hamelin, Eric Trably

Marie-Thérèse Giudici-Orticoni and Saida Benomar participated in the discussions by telephone from Marseilles. Christian Croux and Philippe Soucaille were present at the meeting but are not in the photograph.

There was detailed discussion as follows:

  1. Administrative matters (Eric Trably)
  2. Task 1: presentation of Senthil Kumar Velusamy by Isabelle Meynial-Salles, and discussion of his results
  3. Task 1: presentation of the results of Saida Benomar (by telephone)
  4. Task 2: presentation of the results of Yan Rafrafi
  5. Task 2/3: Presentation of the results of Marianne Quémeneur
  6. Task 4: Presentation of preliminary modelling results (María Luz Cárdenas and Athel Cornish-Bowden)
  7. General discussion, conclusions

Photographie du personnel à Toulouse

4 March 2010. Technical meeting (BIP, LBE) in Marseilles on the molecular analysis for following the consortia.

18 May 2010. Committee meeting (LISBP, BIP, LBE) in Narbonne for the thesis of Yan Rafrafi.

The project logo

InGEcoH logo C. acetobutylicum D. vulgaris

The logo was conceived and executed by Giselle Leroy (BIP). It symbolizes the two species of bacteria to be used in the project: Clostridium acetobutylicum (left) and Desulfovibrio vulgaris (right).