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Stéphane Grimaldi

Welcome to Stéphane Grimaldi’s homepage !

Associate Professor (Maître de Conférences HDR)
Aix-Marseille University - Faculty of Sciences
Email : stephane.grimaldi ”at”
Phone : (00 33) 491-164-557

Elected member of the French National Commitee for Scientific Research
(CoNRS - section 20, mandate 2016-2021)

You can follow me on ResearchGate or LinkedIn

Education :

  • B.Sc. (Maîtrise) Physics 1997, Aix-Marseille Univ.
  • M.Sc. (DEA) Biological Crystallography and NMR 1998, Univ. Paris XI/Orsay
  • Ph.D. (Dr. phil. nat.) Physical chemistry 2002, Univ. Frankfurt
  • Habilitation à Diriger des Recherches 2015, Aix-Marseille Univ.

Academic positions :

  • Wissenschaftlicher Mitarbeiter, 1998-2002, Univ. Frankfurt
  • Post-Doc/lecturer (ATER CNU28) 2002-03, Aix-Marseille Univ.
  • Assistant Professor (CNU 28), Aix-Marseille Univ. since 2003
  • CNRS Research fellow (délégation), 2008-10

Main research interests :

Expertise :

Biomolecular Electron Paramagnetic Resonance (EPR) Spectroscopy

(Former) Students and Post-docs (thanks a lot to them !) :

Post-Docs :

  • Maryam Seif Eddine (ATER) 2017-2019
  • Sinan Al-Attar 2016-2017
  • Julia Rendon 2017 (ATER)
  • Pascal Lanciano 2008 (ATER)

Ph.D :

  • Alexandre Uzel 2018-...
  • Maryam Seif Eddine 2015-2018
  • Julia Rendon 2013-2016
  • Pierre Ceccaldi 2009-2013
  • Rodrigo Arias-Cartin 2006-2010
  • Pascal Lanciano 2004-2008

Post-graduate (M2) :

  • Mélanie Rossotti 2019 (Chemistry)
  • Julia Rendon 2013 (Physics)
  • Zeinab Fahs 2012 (Biochemistry)
  • Mike El Kousseifi 2011 (Physics)
  • Rodolphe Szenes 2010 (Physics)
  • Cédric Schaefer 2009 (Chemistry)
  • Pascal Lanciano 2004 (Biochemistry)

Undergraduate (M1) :

  • Vincent Sirjacques 2018 (Physics)
  • Camille Garcia 2017 (Biochemistry)
  • Julia Rendon 2012 (Physics)
  • Aurélien Fouillen 2011 (Biochemistry)
  • Marion Tardieu 2009 (Physics)
  • Mathieu Colombani 2008 (Physics)
  • Kevin Green 2007 (Physics)
  • Eric Tournayre 2006 (Physics)
  • Mohammed Rida Said Eddine 2005 (Physics)
  • Falk Wachowius 2005 (Biochemistry)
  • Cécile Verny 2003 (Physics)
  • Pascal Lanciano 2003 (Biochemistry)

Undergraduate (L2, L3) :

  • David Busto 2013 (Physics/Chemistry)
  • Armelle Diakese 2012 (Chemistry)

Publications : ((former) students & post-docs are underlined)


20 - M. Seif Eddine, F. Biaso, R. Arias-Cartin, E. Pilet, J. Rendon, S. Lyubenova, F. Seduk, B. Guigliarelli, A. Magalon & S. Grimaldi*
Probing the menasemiquinone binding mode to nitrate reductase A by selective 2H & 15N labelling, HYSCORE spectroscopy and DFT modeling
ChemPhysChem (2017), 18, 2704-2714
Special issue : Physical Chemistry in France

19 - J. Rendon, F. Biaso, P. Ceccaldi, R. Toci, F. Seduk, A. Magalon, B. Guigliarelli & S. Grimaldi*
Elucidating the structures of the low- and high-pH Mo(V) species in respiratory nitrate reductase : A combined EPR, 14,15N HYSCORE, and DFT study
Inorg. Chem. (2017), 56, 4422-4434

18 - R. Arias-Cartin, P. Ceccaldi, B. Schoepp-Cothenet, K. Frick, J.-M. Blanc, B. Guigliarelli, A. Walburger, S. Grimaldi, T. Friedrich, V. Receveur-Bréchot* & A. Magalon*
Redox cofactor insertion in prokaryotic molybdoenzymes occurs via a conserved folding mechanism
Scientific Reports (2016), 6:37743

17 - S. Duval, J. M. Santini, D. Lemaire, F. Chaspoul, M. J. Russel, S. Grimaldi, W. Nitschke & B. Schoepp-Cothenet*
The H-bond network surrounding the pyranopterins modulates redox cooperativity in the molybdenum-bisPGD cofactor in arsenite oxidase
Biochim. Biophys. Acta - Bioenergetics (2016), 1857, 1353-1362

16 - P. Ceccaldi, J. Rendon, C. Léger, R. Toci, B. Guigliarelli, A. Magalon, S. Grimaldi & V. Fourmond*
Reductive activation of E. coli respiratory nitrate reductase
Biochim. Biophys. Acta - Bioenergetics (2015), 1847, 1055-1063

15 - J. Rendon, E. Pilet, Z. Fahs, F. Seduk, L. Sylvi, M. Hajj Chehade, F. Pierrel, B. Guigliarelli, A. Magalon* & S. Grimaldi*
Demethylmenaquinol is a substrate of Escherichia coli nitrate reductase A (NarGHI) and forms a stable semiquinone intermediate at the NarGHI quinol oxidation site
Biochim. Biophys. Acta - Bioenergetics (2015), 1847, 739-747

14 - S. Grimaldi, B. Schoepp-Cothenet, P. Ceccaldi, B. Guigliarelli* & A. Magalon*
The prokaryotic Mo/W-bisPGD enzymes family : a catalytic workhorse in bioenergetics
Biochim. Biophys. Acta - Bioenergetics (2013), 1827, 1048-1085

13 - R. Arias-Cartin, S. Grimaldi, P. Arnoux, B. Guigliarelli & A. Magalon*
Cardiolipin binding in bacterial respiratory complexes : Structural and functional implications
Biochim. Biophys. Acta - Bioenergetics (2012), 1817, 1937-1949

12 - S. Grimaldi*, R. Arias-Cartin, P. Lanciano, S. Lyubenova, R. Szenes, B. Endeward, T. F. Prisner, B. Guigliarelli & A. Magalon
Determination of the proton environment of high stability menasemiquinone intermediate in Escherichia coli nitrate reductase A by pulsed EPR
J. Biol. Chem. (2012), 287, 4662-4670

11 - R. Arias-Cartin, S. Grimaldi, J. Pommier, P. Lanciano, C. Schaefer, P. Arnoux, G. Giordano, B. Guigliarelli & A. Magalon*
Cardiolipin-based respiratory complex activation in bacteria
Proc. Nat. Acad. Sci. USA (2011), 108, 7781-7786

Press release on the CNRS/INSB website (in french) (Comment le cardiolipide assure le bon fonctionnement des complexes respiratoires).

10 - F. MacMillan*, S. Kacprzak, P. Hellwig, S. Grimaldi, H. Michel & M. Kaupp*
Elucidating mechanisms in haem copper oxidases : The high-affinity QH binding site in quinol oxidase as studied by DONUT-HYSCORE spectroscopy and density functional theory
Faraday Discussions (2011), 148, 315-344

9 - R. Arias-Cartin, S. Lyubenova, P. Ceccaldi, T. F. Prisner, A. Magalon, B. Guigliarelli & S. Grimaldi*
HYSCORE evidence that mena- and ubisemiquinones bind at the same Q-site (QD) of E. coli nitrate reductase A
J. Am. Chem. Soc. (2010), 132, 5942-5943

8 - S. Grimaldi*, R. Arias-Cartin, P. Lanciano, S. Lyubenova, B. Endeward, T. F. Prisner, A. Magalon & B. Guigliarelli
Direct evidence for nitrogen ligation to the high-stability semiquinone intermediate in E. coli nitrate reductase A
J. Biol. Chem. (2010), 285, 179-187

7 - P. Lanciano, A. Savoyant, S. Grimaldi, A. Magalon, B. Guigliarelli & P. Bertrand*
A new method for the spin quantitation of [4Fe-4S]1+ clusters with S = 3/2. Application to the FS0 center of the NarGHI nitrate reductase from Escherichia coli
J. Phys. Chem. B (2007), 111, 13632-13637

6 - P. Lanciano, A. Vergnes, S. Grimaldi, B. Guigliarelli & A. Magalon*
Biogenesis of a respiratory complex is orchestrated by a single accessory protein
J. Biol. Chem. (2007), 282, 17468-17474

5 - P. Lanciano, A. Magalon, P. Bertrand, B. Guigliarelli & S. Grimaldi*
High-stability semiquinone intermediate in the nitrate reductase A (NarGHI) from Escherichia coli is located in a quinol oxidation site close to heme bD
Biochemistry (2007), 46, 5323-5329

4 - S. Grimaldi*, P. Lanciano, P. Bertrand, F. Blasco, & B. Guigliarelli
Evidence for an EPR-detectable semiquinone intermediate in the membrane-bound subunit NarI of nitrate reductase A (NarGHI) from Escherichia coli
Biochemistry (2005), 44, 1300-1308

3 - S. Grimaldi, T. Ostermann, N. Weiden, T. Mogi, H. Miyoshi, B. Ludwig, H. Michel, T. F. Prisner & F. MacMillan*
Asymmetric binding of the high-affinity QH.- ubisemiquinone in quinol oxidase (bo3) from E. coli studied by multifrequency electron paramagnetic resonance spectroscopy
Biochemistry (2003), 42, 5632–5639

2 - B. Bouderaux, F. MacMillan, C. Teutloff, R. Agalarov, F. Gu, S. Grimaldi, R. Bittl, K. Brettel & K. Redding*
Mutations in both sides of the photosystem I reaction centre identify the phylloquinone observed by electron paramagnetic resonance spectroscopy
J. Biol. Chem. (2001), 276, 37299-37306

1 - S. Grimaldi, F. MacMillan*, T. Ostermann, B. Ludwig, H. Michel, & T. F. Prisner
QH.- Ubisemiquinone Radical in the bo3-Type Ubiquinol Oxidase Studied by Pulsed Electron Paramagnetic Resonance and Hyperfine Sublevel Correlation Spectroscopy
Biochemistry (2001), 40, 1037 –1043

Book chapters

2 - S. Grimaldi, F. Biaso, B. Burlat, B. Guigliarelli*
Electron paramagnetic resonance studies of molybdenum enzymes
In Molybdenum and Tungsten Enzymes : Spectroscopic and Theoretical Investigations
Edited by Russ Hille, Carola Schulzke & Martin Kirk
Royal Society of Chemistry Metallobiology Series No. 7 (2016), pp. 68-120

1 - S. Grimaldi
Principe de la spectroscopie ENDOR en onde continue
in La spectroscopie de résonance paramagnétique électronique - Applications
edp Sciences, collection Grenoble Sciences (2014), 373-385

Teaching :

Signal Processing :

  • Signal et mesure (L3 Physique-Chimie)
  • Théorie du signal (L3 Physique)
  • Electronique pour l’instrumentation (M1 NanoSciences)
  • Instrumentation (L2 SPI)

Spectroscopy :

  • Spectroscopie, microscopie, imagerie (L2 Sciences de la Vie)
  • Mise en situation Chimie Analytique (M2 Chimie ACS)
  • Caractérisation biophysique (M1 Biochimie BSG)

Physics :

  • Bases de Physique : optique et électricité (L1 Sciences de la Vie)

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